Micro diffusion agar precipitin technique convenient for viewing and recording

Micro diffusion agar precipitin technique convenient for viewing and recording.

Micro gel diffusion reactions, though used extensively in recent years, still present problems in viewing and recording. Direct photographs of these reactions, that usually must be enlarged, are not always satisfactory. Better results are obtained by using the dried, stained film as a negative and printing directly from this. Microscope slides are most often used, but glasses (2 by 2 inches) su...

Photographic recording of precipitin bands in agar gel.

The method for producing antigen-antibody precipitin bands in agar gel, due to Ouchterlony, is becoming increasingly useful in diagnostic immunology as well as in research into antibody-antigen reactions. For both these purposes a dimensionally accurate record of all bands, including faint lines, crossovers, and spurs, is necessary for correct interpretation of the plate. The dimensional accura...

Agar-gel precipitin-inhibition technique for plague antibody determinations.

An application of the agar-gel precipitin-inhibition technique we described previously can be used to detect plague antibodies in human and animal sera after a series of plague vaccine inoculations or after exposure to Pasteurella pestis. Determination of the minimal reacting concentrations of the plague antigen and antibody reagents, methods for combining reagents, and length of incubation per...

Agar-gel precipitin-inhibition technique for histoplasmosis antibody determinations.

The agar-gel precipitin-inhibition technique has been modified to detect antibodies of Histoplasma capsulatum in sera from human clinical cases and experimental animals infected with this organism. By use of this modified technique, the histoplasmosis can be detected more consistently and reliably than with the direct agar-gel diffusion test, and titers are comparable to those attained by the c...

A Useful Application of the Agar Double-Diffusion Technique in the Precipitin Test